Journal: STAR Protocols

Article Title: Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

doi: 10.1016/j.xpro.2024.103038

Figure Lengend Snippet:

Article Snippet: Incubate for 10 min at 4°C. k. Add 90 μL of thawed (frozen at −80°C) antibody cocktail, resuspend and incubate at 4°C for 30 min. l. Wash twice with 1 mL CSB and centrifuge at 600 × g (4°C) for 5 min. Aspirate the supernatant carefully. m. For the fixation step, gently resuspend cell pellet in 500 μL of freshly prepared 2% methanol-free formaldehyde (FA) solution (Pierce, freshly diluted from stock solution in PBS) (max. of 1 Mio cells per 100 μL FA solution, rotate with a volume bigger than 500 μL) and incubate overnight at 4°C.

Techniques: Recombinant, Labeling, Blocking Assay, Software, Cytometry, Flow Cytometry, Single-cell Analysis, Gene Expression